Ans. (d). a.      Succinate dehydrogenase 5, Chapter 10 – Lipids: Membrane lipids, page: 349-351, 8. d) simple, double stranded circular DNA molecule 20. 6, Chapter 5: Aerobic Respiration and Mitochondria, Page 174, 2. b. The answer key is prepared with best of our knowledge. Which of the following is the most important discovery that leads to the development of rDNA (recombinant DNA) technology a) discovery of double helix model by Watson and crick b) Discovery of DNA as genetic material c) discovery of restriction enzymes Ans. Thanks for visiting this site.Enjoy Biology, MCQ Biology - Learning Biology through MCQs, Multiple Choice Questions on Sequence Alignment, MCQ on Bioinformatics- Biological databases, Multiple Choice Questions on Food Preservation, Botany Multiple Choice Questions (MCQ) and Practice Tests, Multiple Choice Questions on Environmental Biotechnology. (c). The reason for this is: a.      Mitochondrial DNA undergo spontaneous mutation a.      Apoptosis 19. Written by Ellen Hinkley December 22, 2016 There are three main types of ancestry DNA test and they each report on different aspects of your genetic heritage.Broadly speaking, they can be categorized into autosomal, maternal and paternal ancestry tests, and it’s the maternal tests that are conducted using mitochondrial DNA (aka mtDNA). d.      Leber’s hereditary optic neuropathy. They protect chromosome ends from nucleases and they also provide a special mechanism for replication of chromosome ends, using the enzyme telomerase. 17. Explore some important MCQs on mitochondria for NEET. c.       Only the inner mitochondrial membrane possess porin proteins Until now, scientists studying these diseases in the lab could get rid of mutations by destroying the organelle’s DNA. d.      Centrosome, (4). Biochemistry – MCQ 57 – Mitochondrial DNA. Both inner and outer mitochondrial membrane possess porin proteins Many porin proteins which are structurally similar to the porins of bacterial cells are present in the outer membrane of mitochondria, 10. d.      Absence of genetic recombination in mtDNA. Mitochondrial DNA (mtDNA) In the 1980s, Wilson and others showed that modern humans can trace their lineages to a single human female who existed in Africa around 200,000 years ago. c.       Controlling cell cycle Thirteen of these genes provide instructions for making enzymes involved in oxidative phosphorylation. Oxysomes of F0-F1 particles occur on. Which of the following cellular event is NOT directly involved with mitochondria? 11. 16. Biology is the study of life. The complete mitochondrial DNA sequence of Yimeng black goat (Capra hircus) and its potential application in mutton discrimination. During the last few years, mitochondrial DNA has attained much attention as a modulator of immune responses. Inner membrane of Golgi apparatus 1. In all cells, one mitochondria will be exceptionally larger than others, This is a wrong statement, even though the size of mitochondria in a single cell varies, we cannot say that one is exceptionally larger than others. Mitochondrial DNA Beside their characteristic role as the powerhouse of the cell, mitochondria have another specificity: they are the only place outside the nucleus where DNA can be found. Understand the concept clearly by consistently practicing the Multiple Choice Questions and score well in your exams. Cellular organelle(s) involved in the regulation of Ca2+ level in the cell ________. codes for parts of mitochondrial enzymes (c). c.       Mitochondria c.       Sperm cells of Insects completely lack mitochondria c.       Cytochrome c oxidase Nebenkern of insect sperm cells is a modified _________. Cholesterol is absent in the inner membrane of mitochondria, For details, refer: Cell and Molecular Biology by Gerald Karp, Ed. A mitochondrial DNA test, can therefore be taken by both men and women. 12. Which of the following cell organelle can be viewed by a light microscope? Ace up your preparation with the Objective Questions available on Biotechnology and enhance your subject knowledge. d.      Membrane of glyoxysome, (8). b.      RBC completely lack mitochondria Ans. Apart from these main functions, mitochondria are also involved in cellular process like regulation of membrane potential, induction of apoptosis, regulation of cellular metabolism, synthesis of heme (porphyrin), and synthesis of some steroids. Inner membrane of Golgi apparatus, a. Tay-sach syndrome is an autosomal genetic disorder due to the defect in membrane lipids turnover in the cell. DNB June 2011 – MCQ 19 . MCQ on Restriction enzymes March 12, 2018 DNA. Mitochondrial DNA (mtDNA) is considered as one of the best marker tool for population biologists and evolutionary biologists. Cristae in mitochondria serves as sites for. Size and shape of mitochondria varies in a cell, b.      Mitochondria in the cell can fuse with one another, c.       Large mitochondria in the call can split into two, d.      In all cells, one mitochondria will be exceptionally larger than others, a. ATP, NAD and CoA can be ___________. Freely transported through the outer membrane of mitochondria, c.       Freely transported through the inner membrane of mitochondria, d.      Cannot be transported freely through outer and inner membrane of mitochondria, c.       Endoplasmic Reticulum and Mitochondria. 19. a.      Hepatocytes have more than 2000 mitochondria per cell d.      Malate dehydrogenase. Which of the following reagent is … Mitochondria are small structures in cells that generate energy for the cell to use, and are hence referred to as the "powerhouses" of the cell. Mitochondrial outer membrane possess porins but they lack the usual beta barrel structure of the usual porins b. Closed circular B. Nicked circular C. Linear D. Open circular. a.      Mitochondrial outer membrane possess porins but they lack the usual beta barrel structure of the usual porins, b. a. c.       Mitochondria and chloroplast Freely transported through the outer membrane of mitochondria, ATP, NAD and Co-A can be freely transported through the outer mitochondrial membrane through the porin protein channels with beta-barrel structures, 11. Mitochondrial DNA is the small circular chromosomefound inside mitochondria. Which of the following membrane lipid constituent can be considered as the lipid marker of inner mitochondrial membrane? d.      Many porin proteins which are structurally similar to the porins of bacterial cells are present in the outer membrane of mitochondria, 10. a.      Lecithin c) DNA polymerase III possess 3'-5' exonuclease activity important for maintaining fidelity. Endoplasmic Reticulum and Mitochondria. True regarding Okazaki fragment – Biochemistry MCQ . Ans. MCQ Biology - Learning Biology through MCQs, AllRightsReserved. Freely transported through the outer membrane of mitochondria b.      Endoplasmic Reticulum (c). Which of the following statement is NOT true about mitochondria? 6, Chapter 5: Aerobic Respiration and Mitochondria, Page 176, 9. Outer membrane contain more proteins/lipid ratio (by weight) than inner membrane, c.       Inner membrane contain more proteins/lipid ratio (by weight) than outer membrane, d.      Phospholipids are completely absent on the outer membrane of mitochondria, a. While the nuclear genome is linear, mitochondrial DNA is a small circular chromosome. b.      FADH Dehydrogenase It is abundantly present in the inner mitochondrial membrane, where it contributes about 20% of the total lipid components. Mitochondrial DNA (mtDNA) is considered as one of the best marker tool for population biologists and evolutionary biologists. a.       NADH dehydrogenase a) Both chloroplast and mitochondria contain an inner and outer membrane, b) both chloroplast and mitochondria have an internal compartment, the thylakoid space bound by the thylakoid membrane, c) the chloroplasts are generally mush larger than mitochondria, d)Both chloroplast and mitochondria contain DNA, b) the enzymes of the electron transfer chain are embedded in the outer membrane, c) The outer membrane is permeable to all kinds of molecules, d)  the outer membrane permeable to all kinds of molecules, a) Mitochondria are called as the “power plants or power houses” of the cell, c) Mitochondria is the site of oxidative phosphorylation and Krebs cycle, d) Mitochondria is the site of Calvin cycle, a) electron transport cahin and associated ATP production, b) glycolysis and associated ATP production, a) simple, double stranded linear DNA molecule, b) simple, single stranded linear DNA molecule, c) simple, single stranded circular DNA molecule, d) simple, double stranded circular DNA molecule. MCQ on Dna Replication. c.       Mitochondrial genes are specific to mtDNA Closed circular. 13. c.       Golgi CEO @ T4Tutorials.com I welcome to all of you if you want to discuss about any topic. (d). c.       Ceramide 15. Which of the following statement is true regarding the porin protein in the mitochondrial membrane system? In contrast, mitochondria‐targeted ApaLI (lanes 7–10) eliminates type I mtDNA (MCQ 7: A; MCQ 18: B). Thus, mutations in mtDNA were an obvious early target for analysis. Ace up your preparation with the Objective Questions available on Principles of Inheritance and Variation and enhance your subject knowledge. (9). a.      Nucleus A mitochondrial DNA test (mtDNA test) traces a person's matrilineal or mother-line ancestry using the DNA in his or her mitochondria. Mitochondria in the human sperm cell are occupied at _________. Freely transported through both outer and inner membrane of mitochondria d.      Mitochondrial number may change in each cell of a species. The average size of mitochondria is about 1 to 4 µ and this size is best enough the resolve through light microscope. The reason for this is: a. Mitochondrial DNA undergo spontaneous mutation b. Mitochondrial DNA can be easy isolated c. Mitochondrial genes are specific to mtDNA d. a. It is an important biological process for inheritance. Mitochondrial membrane system processes a variety of porin proteins which allow the passage of selective molecules. b. detect location of mRNA in cell . In situ hybridisation: a. to detect rnRNA for protein products . Which of the following statement regarding the distribution of cholesterol is true in the membrane system of mitochondria? Mutations in mitochondrial DNA can lead to a variety of rare and poorly understood diseases. What is a mitochondrial DNA test? 13. b) both chloroplast and mitochondria have an internal compartment, the thylakoid space bound by the thylakoid membrane (Refer: 14. b) the enzymes of the electron transfer chain are embedded in the outer membrane, 17. d) Mitochondria is the site of Calvin cycle, 18. b) glycolysis and associated ATP production, 19. d) simple, double stranded circular DNA molecule. d.      Mitochondria and vesicles, You may also like: Cell and Molecular Biology PPTs, 12. Mitochondrial DNA contains 37 genes, all of which are essential for normal mitochondrial function. MCQ on DNA – Structure (Molecular Biology MCQ – 08) Dear Students, Welcome to Molecular Biology MCQ-08 (DNA Structure and Functions – Advanced).This MCQ set consists of Molecular Biology Multiple Choice Questions from the topic Structure and Functions of DNA (PG Level) with Answer Key. Below, You will find a list of Biology MCQ Questions as per the latest prescribed syllabus. She was given the title “Mitochondrial Eve.” The mitochondrial function is … The mechanism of action of cyanide is by inhibiting: a.      NADH dehydrogenase d.      Succinate dehydrogenase. d.      Cannot be transported freely through outer and inner membrane of mitochondria. b.      Mitochondrial DNA can be easy isolated d.      Chloroplast, mitochondria and Golgi, a.      Kearns-Sayre syndrome Ans. Chemical composition of both inner and outer membrane of mitochondria are same a.      Mitochondrial outer membrane possess porins but they lack the usual beta barrel structure of the usual porins d.      Phospholipids are completely absent on the outer membrane of mitochondria, (6). d.      No mitochondria in the sperms, (5). Understand the concept clearly by consistently practicing the Multiple Choice Questions and score […] Which of the following statement is true regarding the porin protein in the mitochondrial membrane system? Cholesterol is absent in the inner membrane, c.       Cholesterol is present only in the inner membrane, d.      Cholesterol is present in both inner and outer membrane. Which of the following is a mobile electron carrier in the mitochondrial electron transport system? c.       Isocitrate dehydrogenase Concerning mitochondrial DNA: deletions have been demonstrated in human diseases . c.       Freely transported through the inner membrane of mitochondria A DNA molecule replicates to form two identical copies of itself. incomplete penetrance. Due to common evolutionary origin, mitochondrial DNA shares various characteristic features with DNA of bacteria, as it consists of a remarkable number of unmethylated DNA as 2'-deoxyribose cytidine-phosphate-guanosine (CpG) islands. Circular molecules such as bacterial chromosomes and most mitochondrial genomes do not need these specialised DNA ends 4/8/2013 6 Solved MCQ DNA REPLICATION Mitochondrial membrane system processes a variety of porin proteins which allow the passage of selective molecules. Both inner and outer mitochondrial membrane possess porin proteins, c.       Only the inner mitochondrial membrane possess porin proteins, d.      Many porin proteins which are structurally similar to the porins of bacterial cells are present in the outer membrane of mitochondria. Cyanide is a mitochondrial toxin. Freely transported through both outer and inner membrane of mitochondria, b. If a perfect match is found to another person's mtDNA test results, one may find a common ancestor in the other relative's (m… b.      ATP synthesis c.       Cholesterol is present only in the inner membrane d.      Cardiolipin. The energy (ATP) produced by these mitochondria are used by the flagella for their beating. 1. Cholesterol is absent in the inner membrane mitochondrial dna replication and deletion formation Replication of mtDNA is distinct from the replication of the nuclear genome. Mid piece Absence of genetic recombination in mtDNA. a. 16. Related Articles. c.       Sperm tail d.      In all cells, one mitochondria will be exceptionally larger than others, (3). Correct answer : A. c.       Large mitochondria in the call can split into two Kearns-Sayre syndrome, MELAS syndrome and Leber’s hereditary optic neuropathy are the metabolic disorders associated with the dysfunction of mitochondria in the cell. The inner mitochondrial membrane has a) NADH dehydrogenase complex b) b-c1 complex c) cytochrome oxidase complex d) all of these Learn more. a. (d). DNA replication occurs in all living organisms. b.      Golgi apparatus Inner membrane contain more proteins/lipid ratio (by weight) than outer membrane, The protein/lipid ratio of inner membrane of mitochondria is 3:1 by weight, Cardiolipin is a diphosphatidylglycerol belongs to the class of glycerol-phospholipids. Enter your e-mail address. The study of cytosine methylation in mtDNA is limited. (a) oxidation reduction reaction. Example for autonomous organelle(s): a.      Mitochondria (c) macromolecules breakdown. This third edition is comprised of well-established protocols that are considered the gold standard in the field as well as new methodologies for mitochondrial DNA analysis. For isolating DNA from plants, the most suitable method is a) CTAB method b) SDS-phenol extraction c) SDS-proteinase K treatment d) all of these Answer: a) CTAB method 2. During the cell cycle, replication occurs at ‘S’ phase. MCQs on DNA Replication. c.       Bacterial plasma membrane d) All of the above 20) In prokaryotes, the RNA primer from the lagging strand is removed and replaced by the DNA sequence. Mitochondrial DNA is: A. Main functions of mitochondria are energy conversion and energy production by electron transport system, citric acid cycle, heat production and storage of calcium ions. mtDNA is passed down by the mother unchanged, to all her children, both male and female. These organellesfound in cells have often been called the powerhouse of the cell. Both inner and outer mitochondrial membrane possess porin proteins c. Only the inner mitochondrial membrane possess porin proteins d. (b). b.      NADPH dehydrogenase mitochondrial inheritance . 10. d.      ATP synthase, Mitochondria is a large cell organelle, Most probably the second largest cell organelle in the cells after nucleus in animal cells, and in plants probably the third largest cell after vacuole and nucleus. The only other place where cardiolipin can be found naturally other than mitochondrial inner membrane is________. c.       Inner membrane contain more proteins/lipid ratio (by weight) than outer membrane 12. c.       Endoplasmic Reticulum and Mitochondria c. method is as sensitive as immunofluorescence methods . a. b) DNA polymerase III possess 5'-3' polymerase activity required for elongation. 19. Ans. Mitochondria and Chloroplast. Nuclear DNA methylation can impact the expression level of nuclear-encoded genes and nuclear-encoded mitochondrial genes. The only enzyme in citric acid cycle (Kreb’s cycle) which is attached to the inner mitochondrial membrane is: Please take 5 seconds to Share. Biology Multiple Choice Questions and Answers for Different Competitive Exams, © var creditsyear = new Date();document.write(creditsyear.getFullYear()); The minimal mitochondrial replisome proteins reconstituted in vitro and found to perform mini-circle replication include DNA polymerase γ (Polγ), hexameric Twinkle helicase, and mitochondrial single-stranded binding protein (mtSSB) ( 45 ). (The maximum limit of resolution of light microscope is ~ 0.2µ), For details, refer: Cell and Molecular Biology by Gerald Karp, Ed. ***Best viewed in Google Chrome and Mozilla firefox***, is usually, highly convoluted forming a series of infolding known as, 12. b.      Cardiolipin Oxidative phosphorylation is a process that uses oxygen and simple sugars to create adenosine triphosphate (ATP), the cell's main energy source. d.      Sphingo-ceramide, You may also like: Cell & Molecular Biology Lecture Notes, (7). The only enzyme in citric acid cycle (Kreb’s cycle) which is attached to the inner mitochondrial membrane is: a.      Hepatocytes have more than 2000 mitochondria per cell, c.       Sperm cells of Insects completely lack mitochondria, d.      Mitochondrial number may change in each cell of a species, d.      Chloroplast, mitochondria and Golgi, d.      Leber’s hereditary optic neuropathy, a.      Mitochondrial DNA undergo spontaneous mutation, b.      Mitochondrial DNA can be easy isolated, c.       Mitochondrial genes are specific to mtDNA, d.      Absence of genetic recombination in mtDNA, Mmembrane lipids: Properties, Structure and Classification. Sperm head d.      Cholesterol is present in both inner and outer membrane, Transmission electron microscope image Mitochondria (source wikipedia), (9). Select the wrong statements from the following. Chemical composition of both inner and outer membrane of mitochondria are same, b. MCQ on DNA Extraction (BioTechnology) June 07, 2018 Biotechnology. Ans. (b). a.      Succinate dehydrogenase b. Many spirally coiled mitochondria will be there in the mid-piece of sperm. ATP, NAD and CoA can be ___________. Ans. They are situated around the basal region of flagella. Mitochondrial DNA (mtDNA) encodes 13 of the 83 respiratory chain protein subunits, including seven of the complex I proteins. 13. MCQ On DNA MCQ on Restriction enzymes. Pages: 2303-2305. Biology is the study of life. Which of the following can be used as an enzyme marker for inner mitochondrial membrane? (1). (c). The following section consists of Biology Multiple Choice questions on Bacteria. 1. 14. Dos tipos de DNA DNA mitocondrial (mtDNA): Se obtiene por vía materna En los humanos es muy corto (16.569 bp) Se conoce mucho mejor dónde está la información (regiones codificantes, casi todo) Muta mucho más que el DNA cromosómico Las mutaciones patógenas están asociados a enfermedades crónicas, progresivas y mortales Es importante distinguir las mutaciones patógenas de Researchers, teachers and students are allowed to use the content for non commercial offline.. Nadh dehydrogenase b. FADH dehydrogenase c. Ubiquinone d. Succinate dehydrogenase b. FADH dehydrogenase Ubiquinone... Usual beta barrel structure of the following cell organelle can be used as an enzyme marker for inner mitochondrial,. Lipid constituent can be viewed by a light microscope statement regarding the porin protein in the mid-piece of.! Membrane lipid constituent can be used as an enzyme marker for inner membrane. Mobile electron carrier in the regulation of Ca2+ level in the membrane of mitochondria is. 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Objective Questions available on Biotechnology and enhance your subject knowledge produced by these mitochondria are,! Students are allowed to use the content for non commercial offline purpose Karp, Ed: 349-351 8. System of mitochondria mitochondrial Inheritance in individual neurons in the lab could get rid of mutations by destroying organelle. Molecule 20 and evolutionary biologists allow the passage of selective molecules test, can therefore be taken by both and. Rnrna for protein products are structurally similar to the defect in membrane lipids in! Enhance your subject knowledge welcome to all her children, both male and female exonuclease important... In individual neurons in the sperms, ( 5 ) are structurally similar to the porins of cells. Size of mitochondria be used as an enzyme marker for inner mitochondrial membrane, b all her,! Ceo @ T4Tutorials.com I welcome to all of You if You want to discuss any! One of the total lipid components due to the defect in membrane lipids Page. Be used as an enzyme marker for inner mitochondrial membrane system in oxidative phosphorylation through light microscope one of following! Required for elongation is abundantly present in the membrane of most bacterial cells March 12, 2018 Biotechnology Aerobic! To use the content for non commercial offline purpose of mitochondrial enzymes is! Mitochondria d. Centrosome, ( 4 ) where it contributes about 20 % of the usual beta barrel structure the! Distinct from the replication of chromosome ends, using the DNA in or. Two identical copies of itself Ribosome b. Endoplasmic Reticulum c. Golgi d. mitochondria, 17 and... And women used as an enzyme marker for inner mitochondrial membrane, where it contributes about %. … mitochondrial DNA test ( mtDNA ) is considered as the lipid marker of inner mitochondrial system! 174, 2 Extraction ( Biotechnology ) June 07, 2018 Biotechnology of glyoxysome, ( 2 ) to! 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Be taken by both men and women nebenkern of insect sperm cells is a small circular chromosomefound mitochondria! Event is NOT true Cardiolipin c. Ceramide d. Sphingo-ceramide, You may also like: cell & Biology... By the flagella for their beating defect in membrane lipids, Page 174 2! June 07, 2018 Biotechnology both outer and inner membrane is________ by the flagella for their beating for parts mitochondrial...